| 1 | 636 | Brňák | Jan | 1996 | M | LAWI STARS - GIANT | | | | 1 | 02:12:04 | 36.8 |
| 2 | 618 | Fiala | Petr | 1995 | M | INTEGRAY L27 CYCLING TEAM | | | | 1 | 02:12:05 | 36.8 |
| 3 | 610 | Přenosil | Marek | 2007 | M | SPORTRACES CYCLING TEAM | | | | 1 | 02:12:08 | 36.8 |
| 4 | 625 | Kubelka | Adam | 2005 | M | ELKOV AUTHOR CYCLING TEAM | | | | 1 | 02:12:14 | 36.8 |
| 5 | 1 | Gdula | Tomáš | 1997 | M | INTEGRAY L27 CYCLING TEAM | UAC | 1 | A | 1 | 02:12:15 | 36.7 |
| 6 | 608 | Saidl | Filip | 2004 | M | MAPEI MERIDA KAŇKOVSKÝ | | | | 1 | 02:12:17 | 36.7 |
| 7 | 637 | Kohout | Martin | 1998 | M | AC SPARTA PRAHA | | | | 1 | 02:12:18 | 36.7 |
| 8 | 627 | Knap | Ondřej | 1995 | M | KUPKOLO.CZ | | | | 1 | 02:12:21 | 36.7 |
| 9 | 631 | Leffler | Kryštof | 2006 | M | KOVO PRAHA | | | | 1 | 02:12:23 | 36.7 |
| 10 | 633 | Kopecký | Štěpán | 2002 | M | LAWI STARS - GIANT | | | | 1 | 02:12:34 | 36.7 |
| 11 | 632 | Purnoch | Matyáš | 2006 | M | REXONIX KOVO PRAHA | | | | 1 | 02:12:37 | 36.6 |
| 12 | 605 | Miklošín | Matyáš | 2005 | M | SPORTRACES CYCLING TEAM | | | | 1 | 02:15:03 | 36 |
| 13 | 634 | Hezina | Jan | 2005 | M | AC SPARTA PRAHA | | | | 1 | 02:15:26 | 35.9 |
| 14 | 607 | Davies | Joshua | 1997 | M | RAFKARNA IDEA CYCLING TEAM | | | | 1 | 02:17:05 | 35.5 |
| 15 | 638 | Bruckner | Filip | 2006 | M | REXONIX KOVO PRAHA | | | | 1 | 02:19:46 | 34.8 |
| 16 | 738 | Bartoš | Karel | 2006 | M | SPORTRACES CYCLING TEAM | | | | 1 | 02:19:46 | 34.8 |
| 17 | 736 | Purnoch | Jan | 2008 | M | REXONIX KOVO PRAHA | | | | 1 | 02:23:26 | 33.9 |
| 18 | 17 | Liemessing | Cedric | 1997 | M | | UAC | 17 | A | 1 | 02:28:35 | 32.7 |
| 19 | 622 | Šurík | Jakub | 2005 | M | KCL KOOPERATIVA - SVIJANY | | | | 1 | 02:29:40 | 32.5 |
| 20 | 628 | Kalauz | Petr | 2008 | M | SPORTRACES CYCLING TEAM | | | | 1 | 02:29:45 | 32.5 |
| 21 | 616 | Horyna | Vlastimil | 2005 | M | SPORTRACES CYCLING TEAM | | | | 1 | 02:35:25 | 31.3 |
| 22 | 629 | Pírek | Ondřej | 1999 | M | | | | | 1 | 03:52:51 | 20.9 |
| 23 | 612 | Vajčner | Jiří | 1997 | M | | | | | 1 | 03:52:52 | 20.9 |
| 24 | 601 | Ondráček | David | 1999 | M | | | | | 1 | 03:52:53 | 20.9 |
| 25 | 639 | Hanzal | Jan | 1997 | M | | | | | 0 | DNF | |
| 26 | 600 | Šafář | Martin | 2005 | M | | | | | 0 | DNF | |
| 27 | 626 | Řezník | Šimon | 2000 | M | AC SPARTA PRAHA | | | | 0 | DNF | |
| 28 | 609 | Nágr | Ondřej | 2007 | M | SPORTRACES CYCLING TEAM | | | | -1 | DNS | |